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By Dominici F., Zeger S. L.

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Liu, C. , Croce, C. , and Harris, C. C. (2006). Unique miRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell. 9(3), 189–198. , Pasolli, H. , Dietrich, F. , and Fuchs, E. (2006). Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs. Nature Genet. 38, 356–362. Zamore, P. , and Haley, B. (2005). Ribo‐gnome: The big world of small RNAs. Science 309, 1519–1524. [3] Troubleshooting Microarray Hybridizations By BRIAN EADS, AMY CASH, KEVIN BOGART, JAMES COSTELLO , and JUSTEN ANDREWS Abstract Microarray experiments are being performed more widely than ever before, but even seasoned investigators can experience technical problems with hybridizations.

2J) can reveal dye, print‐tip, or subarray effects. Data with more pronounced bias will generally require more aggressive normalization due to dye effects that do not reflect underlying transcript levels. Graphical representations of array foreground and background levels, often called heat maps, are another useful way to visualize the common effect of location‐dependent background. First, spatial (x–y) plots of foreground and background across the slide may highlight spatial trends. Spatial plots of ratios (Fig.

Acad. Sci. USA 101, 9740–9744. , Miska, E. , Ebert, B. , Mak, R. , Ferrando, A. , Downing, J. , Horvitz, H. , and Golub, T. R. (2005a). MicroRNA expression profiles classify human cancers. Nature 435, 834. , and Cardoso, W. V. (2005b). Differential expression of components of the microRNA machinery during mouse organogenesis. Biochem. Biophys. Res. Commun. 334, 319–323. , and Borkhardt, A. (2004). High expression of precursor microRNA‐155/BIC RNA in children with Burkitt lymphoma. Genes Chromosomes Cancer 39, 167–169.

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